{"id":30858,"date":"2019-10-31T21:37:49","date_gmt":"2019-10-31T18:37:49","guid":{"rendered":"https:\/\/prohoster.info\/blog\/pervyj-kompyuternyj-genom-mozhet-privesti-k-sozdaniyu-sinteticheskih-form-zhizni\/"},"modified":"2019-10-31T21:37:49","modified_gmt":"2019-10-31T18:37:49","slug":"pervyj-kompyuternyj-genom-mozhet-privesti-k-sozdaniyu-sinteticheskih-form-zhizni","status":"publish","type":"post","link":"https:\/\/prohoster.info\/sq\/blog\/novosti-interneta\/pervyj-kompyuternyj-genom-mozhet-privesti-k-sozdaniyu-sinteticheskih-form-zhizni","title":{"rendered":"Gjenomi i par\u00eb kompjuterik mund t\u00eb \u00e7oj\u00eb n\u00eb krijimin e formave sintetike t\u00eb jet\u00ebs","gt_translate_keys":[{"key":"rendered","format":"text"}]},"content":{"rendered":"

T\u00eb gjitha sekuencat e ADN-s\u00eb s\u00eb organizmave t\u00eb studiuara ruhen n\u00eb nj\u00eb databaz\u00eb q\u00eb i p\u00ebrket Qendr\u00ebs Komb\u00ebtare t\u00eb Informacionit Bioteknologjik n\u00eb SHBA. Dhe m\u00eb 1 Prill, n\u00eb databaz\u00eb u regjistrua nj\u00eb sh\u00ebnim i ri: «Caulobacter ethensis-2.0». Ky \u00ebsht\u00eb gjenomi artificial i par\u00eb n\u00eb bot\u00eb, i simuluar plot\u00ebsisht n\u00eb kompjuter dhe m\u00eb pas i sintetizuar, i nj\u00eb organizmi t\u00eb gjall\u00eb, i zhvilluar nga shkenc\u00ebtar\u00ebt e ETH Zurich (Shkolla Politeknike e Zurichut). Megjithat\u00eb, \u00ebsht\u00eb e r\u00ebnd\u00ebsishme t\u00eb theksohet se, megjith\u00ebse gjenomi C. ethensis-2.0 u arrit si nj\u00eb molekul\u00eb e madhe ADN-je, organizmi i gjall\u00eb i p\u00ebrshtatsh\u00ebm ende nuk ekziston.<\/p>\n

\"Gjenomi<\/p>\n

Pjesa k\u00ebrkimore u krye nga Bit Kristen, profesor i biologjis\u00eb eksperimentale sistemore, dhe v\u00ebllai i tij, Matthias Kristen \u2013 kimist. Gjenomi i ri, i quajtur Caulobacter ethensis-2.0, u krijua duke pastruar dhe optimizuar kodin natyror t\u00eb bakterit Caulobacter crescentus \u2013 nj\u00eb bakter i pad\u00ebmsh\u00ebm q\u00eb jeton n\u00eb uj\u00ebra t\u00eb fresk\u00ebta n\u00eb t\u00eb gjith\u00eb bot\u00ebn.  <\/p>\n

\"Gjenomi
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M\u00eb shum\u00eb se nj\u00eb dekad\u00eb m\u00eb par\u00eb, nj\u00eb ekip n\u00ebn drejtimin e gjenetik\u00ebs Craig Venter krijoi bakterin e par\u00eb \u201csintetik\u201d. Gjat\u00eb pun\u00ebs s\u00eb tij, shkenc\u00ebtar\u00ebt sintetizuan nj\u00eb kopje t\u00eb gjenomit t\u00eb Mycoplasma mycoides, e cila m\u00eb pas u implantua n\u00eb nj\u00eb qeliz\u00eb mbajt\u00ebse, e cila pas k\u00ebsaj doli t\u00eb ishte plot\u00ebsisht funksionale dhe ruajti aft\u00ebsin\u00eb p\u00ebr t\u00eb riprodhuar.<\/p>\n

Studimi i ri vazhdon pun\u00ebn e Kreigerit. N\u00ebse m\u00eb par\u00eb shkenc\u00ebtar\u00ebt krijuan nj\u00eb model digjital t\u00eb ADN-s\u00eb s\u00eb nj\u00eb organizmi t\u00eb v\u00ebrtet\u00eb dhe mbi t\u00eb sintetizuan nj\u00eb molekul\u00eb, projekti i ri shkon m\u00eb larg, duke p\u00ebrdorur kodin origjinal t\u00eb ADN-s\u00eb. Shkenc\u00ebtar\u00ebt e rishikuan at\u00eb ndjesh\u00ebm p\u00ebrpara se t\u00eb sintetizojn\u00eb dhe testojn\u00eb funksionalitetin e saj.<\/p>\n

Shkenc\u00ebtar\u00ebt filluan me gjenomin origjinal t\u00eb C. crescentus, q\u00eb p\u00ebrmban 4000 gene. Ashtu si n\u00eb \u00e7do organiz\u00ebm t\u00eb gjall\u00eb, shumica e k\u00ebtyre geneve nuk mbajn\u00eb asnj\u00eb informacion dhe p\u00ebrb\u00ebjn\u00eb «ADN t\u00eb mbeturinave». Pas analiz\u00ebs, shkenc\u00ebtar\u00ebt arrit\u00ebn n\u00eb p\u00ebrfundimin se vet\u00ebm rreth 680 prej tyre jan\u00eb t\u00eb nevojsh\u00ebm p\u00ebr mbajtjen n\u00eb jet\u00eb t\u00eb bakterit n\u00eb laborator.<\/p>\n

Pas largimit t\u00eb «ADN-s\u00eb s\u00eb mbeturinave» dhe marrjes s\u00eb gjenomit minimal t\u00eb C. crescentus, ekipi vazhdoi pun\u00ebn. ADN-ja e organizmave t\u00eb gjall\u00eb karakterizohet nga nj\u00eb tepric\u00eb e integruar, ku sintetizimi i t\u00eb nj\u00ebjtit protein kodihet nga gene t\u00eb ndryshme n\u00eb disa pjes\u00eb t\u00eb zinxhirit. Shkenc\u00ebtar\u00ebt z\u00ebvend\u00ebsuan m\u00eb shum\u00eb se 1\/6 e 800,000 shkronjave t\u00eb ADN-s\u00eb gjat\u00eb optimizimit duke hequr kodin e dublikuar.<\/p>\n

«Fal\u00eb algoritmit ton\u00eb, ne e shkruam p\u00ebrfundimisht gjenomin n\u00eb nj\u00eb sekuenc\u00eb t\u00eb re shkronjash ADN-je, e cila nuk \u00ebsht\u00eb m\u00eb e ngjashme me origjinalin», thot\u00eb Bit Kristen, bashkautor i studimit. «Megjithat\u00eb, funksioni biologjik n\u00eb nivelin e sintetizimit t\u00eb proteinave mbeti i pacakontestuar».<\/p>\n

P\u00ebr t\u00eb verifikuar n\u00ebse zinxhiri i marr\u00eb do t\u00eb funksionoj\u00eb si\u00e7 duhet n\u00eb nj\u00eb qeliz\u00eb t\u00eb gjall\u00eb, shkenc\u00ebtar\u00ebt rrit\u00ebn nj\u00eb stam bakteresh, t\u00eb cilat kishin n\u00eb ADN-n\u00eb e tyre si gjenomin natyror Caulobacter ashtu edhe segmente t\u00eb gjenomit artificial. Shkenc\u00ebtar\u00ebt \u00e7aktivizuan gene natyror\u00eb t\u00eb ve\u00e7ant\u00eb dhe kontrolluan aft\u00ebsin\u00eb e analog\u00ebve t\u00eb tyre artificial\u00eb p\u00ebr t\u00eb p\u00ebrmbushur t\u00eb nj\u00ebjtin rol biologjik. Rezultati ishte mjaft mbres\u00ebl\u00ebn\u00ebs: rreth 580 nga 680 gene artificial\u00eb ishin funksional\u00eb.<\/p>\n

«Fal\u00eb njohurive t\u00eb fituara, ne do t\u00eb jemi n\u00eb gjendje t\u00eb p\u00ebrmir\u00ebsojm\u00eb algoritmin ton\u00eb dhe t\u00eb zhvillojm\u00eb nj\u00eb version t\u00eb ri t\u00eb gjenomit 3.0», thot\u00eb Kristen. «Mendojm\u00eb se n\u00eb t\u00eb ardhmen e af\u00ebrt do t\u00eb krijojm\u00eb qeliza bakteriale t\u00eb gjalla me nj\u00eb gjenom plot\u00ebsisht sintetik».<\/p>\n

N\u00eb faz\u00ebn e par\u00eb, k\u00ebto k\u00ebrkime do t\u00eb ndihmojn\u00eb gjenetik\u00ebt t\u00eb verifikojn\u00eb sakt\u00ebsin\u00eb e njohurive t\u00eb tyre mbi kuptimin e ADN-s\u00eb dhe rolin e geneve t\u00eb ve\u00e7anta n\u00eb t\u00eb, pasi \u00e7do gabim n\u00eb sintetizimin e zinxhir\u00ebve do t\u00eb \u00e7oj\u00eb n\u00eb nj\u00eb organiz\u00ebm me gjenom t\u00eb ri q\u00eb do t\u00eb vdes\u00eb ose do t\u00eb jet\u00eb jo funksional. N\u00eb t\u00eb ardhmen, ato do t\u00eb \u00e7ojn\u00eb n\u00eb krijimin e mikroorganizmave sintetik\u00eb, t\u00eb cilat do t\u00eb krijohen p\u00ebr q\u00ebllime t\u00eb paracaktuar. Virus\u00ebt artificial\u00eb do t\u00eb mund t\u00eb luftojn\u00eb me t\u00eb af\u00ebrmit e tyre natyror\u00eb, nd\u00ebrsa bakterit\u00eb specifike do t\u00eb prodhojn\u00eb vitamina ose medikamente.<\/p>\n

Studimi u publikua n\u00eb revist\u00ebn PNAS.<\/p>\n

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Burimi: 3dnews.ru<\/a><\/p>","protected":false,"gt_translate_keys":[{"key":"rendered","format":"html"}]},"excerpt":{"rendered":"

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Dhe m\u00eb 1 prill n\u00eb k\u00ebt\u00eb baz\u00eb t\u00eb dh\u00ebnash u shfaq nj\u00eb regjist\u00ebr i ri: \u00abCaulobacter ethensis-2.0\u00bb. Ky \u00ebsht\u00eb gjenomi i par\u00eb n\u00eb bot\u00eb i modelluar plot\u00ebsisht n\u00eb kompjuter dhe m\u00eb pas i sintetizuar, i nj\u00eb organizmi t\u00eb gjall\u00eb, i zhvilluar nga shkenc\u00ebtar\u00ebt e ETH Zurich (Shkolla e Lart\u00eb Teknike e C\u00fcrichut). 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\u0421\u0428\u0410. \u0418 1 \u0430\u043f\u0440\u0435\u043b\u044f \u0432 \u0431\u0430\u0437\u0435 \u0434\u0430\u043d\u043d\u044b\u0445 \u043f\u043e\u044f\u0432\u0438\u043b\u0430\u0441\u044c \u043d\u043e\u0432\u0430\u044f \u0437\u0430\u043f\u0438\u0441\u044c: \u00abCaulobacter ethensis-2.0\u00bb. \u042d\u0442\u043e \u043f\u0435\u0440\u0432\u044b\u0439 \u0432 \u043c\u0438\u0440\u0435 \u043f\u043e\u043b\u043d\u043e\u0441\u0442\u044c\u044e \u0441\u043c\u043e\u0434\u0435\u043b\u0438\u0440\u043e\u0432\u0430\u043d\u043d\u044b\u0439 \u043d\u0430 \u043a\u043e\u043c\u043f\u044c\u044e\u0442\u0435\u0440\u0435 \u0438 \u0437\u0430\u0442\u0435\u043c \u0441\u0438\u043d\u0442\u0435\u0437\u0438\u0440\u043e\u0432\u0430\u043d\u043d\u044b\u0439 \u0441\u0438\u043d\u0442\u0435\u0442\u0438\u0447\u0435\u0441\u043a\u0438\u0439 \u0433\u0435\u043d\u043e\u043c \u0436\u0438\u0432\u043e\u0433\u043e \u043e\u0440\u0433\u0430\u043d\u0438\u0437\u043c\u0430, 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